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. 2000 Aug 21;192(4):463–474. doi: 10.1084/jem.192.4.463

Figure 1.

Figure 1

(A) Northern blot analysis of liver RNA from wild-type (line 30) and transgenic founder mice (lines 33, 22, and 45). (B) Western analysis of OPG from serum and liver extract of line 22 transgenic mice (+) or wild-type mice (−). Chinese hamster ovary cell–produced recombinant OPG (CHO-muOPG) is used as the positive control. (C) Hematoxylin and eosin–stained sections of femoral diaphysis from 6-wk-old wild-type (normal), low (HECR-45), moderate (HECR-33), and high (HECR-22) OPG-expressing F1 transgenic mice. Bone stains pink, and marrow cells stain dark blue. Green boxes represent the area used for histomorphometric quantitation of bone volume. (D) Bone volume as a percentage of tissue volume measurements were made in 6-wk-old femoral diaphysis 2–4 mm from the distal growth plate, and did not include cortical bone (see C–F, above). Values are mean ± SEM, n = 4–7 for F1 OPG transgenics and n = 16 for aged-matched littermates. (E) Osteoclast number per millimeter of growth plate length and active osteoclast surface as a percentage of growth plate length measurements were taken in the femoral metaphysis adjacent to the growth plate from 6-wk-old wild-type, low, moderate, and high OPG-expressing F1 mice. Osteoclasts considered for measurement were TRAP+ cells in contact with a bone surface and within three cell widths of the growth plate.