Table 1.

HMG-1 Activates Cytokine Synthesis in C3H/HeJ Macrophages

[HMG-1]	TNF-producing cells
μg/ml	%
0	0.5
1	5.8
10	19.2
100	4.8

Resident peritoneal macrophages were obtained by peritoneal lavage from C3H/HeJ mice, and cultured for 2 h with rHMG-1 or vehicle in the doses shown. Cells were harvested, fixed on adhesive glass slides, and stained for intracellular TNF using indirect immunofluorescence. Results are based on means of 2,000 counted macrophages on each microscopy slide (in duplicate).