Figure 1.

Migration of LCs out of ear skin explants of CCR2^−/− and CCR5^−/− mice is reduced. (A) The absolute numbers of I-A^b+DEC-205⁺ (LCs) and I-A^b+DEC-205⁻ cells emigrating out of ear skin explants was determined after 24 and 72 h of culture. The data for the 24- and 72-h time points are from one of two and one of three representative experiments, respectively. The migrating I-A^b−Ly-6G⁺ (neutrophils) and CD3⁺CD4⁺ (CD4⁺ T cells) cells were determined after 24 h of culture (one of three representative experiments is shown). Data from skin explants of three to seven mice per group were pooled for analysis. The percentages of I-A^b+DEC-205⁺, I-A^b+DEC-205⁻, I-A^b−Ly-6G⁺, and CD3⁺CD4⁺ cells among the total number of cells that emigrated are shown in parentheses. (B) Representative FACS^® profile showing a marked decrease in I-A^b+DEC-205⁺ cells that migrated out of ear explants of CCR2^−/− and CCR5^−/− mice. (C) The I-A^b+DEC-205⁺ cells (mean number ± SD) present in epidermal cell suspensions was determined by flow cytometry. Epidermal cell suspensions were prepared from three mice per group. Data are from one of two experiments with similar results.