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. 2000 Jul 17;192(2):151–158. doi: 10.1084/jem.192.2.151

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cytokine production in Smad7 Tg mice. OVA-sensitized mice were challenged with inhalation of OVA. At 48 h after the inhalation, BALF was collected as described in Materials and Methods. For detection of cytokines in the culture supernatant, spleen cells were isolated from the mice sensitized with OVA/alum and stimulated with OVA (100 μg/ml) in vitro. 72 h after the stimulation, the culture supernatants were collected. IL-5 and IFN-γ levels in the BALF (A), OVA-induced IL-4, IL-5, and IFN-γ production in the supernatant of spleen cells from the sensitized mice (B), and TGF-β1 levels in the BALF (C) were determined by ELISA. Data are means ± SD for five mice in each group. The results are from one representative experiment out of three. *P < 0.05, significantly different from the mean value of the corresponding control response (Wt).