Figure 1.
Specific association of M. avium with CD43-transfected HeLa cells. HeLa cells transfected with CD43 (HeLa-CD43) and vector-transfected cells (HeLa) were incubated as indicated with stained live M. avium, S. typhimurium, and S. flexneri at 10 organisms/cell for 4 h. Cells were washed, and the infected cells were counted using fluorescence microscopy (see Materials and Methods). Shown is a representative experiment. The number of CD43-transfected HeLa cells that scored positive for M. avium binding was significantly different than the number of vector-transfected cells (n = 3, P < 0.0001).