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. 2000 Sep 5;192(5):761–768. doi: 10.1084/jem.192.5.761

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JEM000325.f2ai.jpg — CCR9 expression is highly specific for CD4⁺ and CD8⁺ lymphocytes infiltrating the small intestine. FACS^® analysis reveals that CCR9 is expressed on virtually all CD4⁺ and CD8⁺ lymphocytes in the (A) IEL and (B) LPL compartments of the jejunum. Interestingly, CCR9 was not detected on lymphocytes isolated from (C) normal lung, (D) hepatitis C virus (HCV)-infected liver, (E) breast milk, (F) normal skin, (G) inflamed skin (delayed-type hypersensitivity [DTH]), (H) rheumatoid arthritis (RA) synovium, and (I) rheumatoid arthritis synovial fluid, and (J) a smaller percentage of colon lymphocytes expresses CCR9 compared with jejunal lymphocytes (2,000 cells in each panel). (A–I) Isotype-matched control Ab staining shown as dotted lines. Percentage of CCR9⁺ cells based on a marker encompassing 5% of isotype control–stained cells in both histogram and dot blots. Data are representative of three jejunum, four colon, four liver, two breast milk, four normal skin, four inflamed skin, four synovium, and four synovial fluid samples.

graphic file with name JEM000325.f2j.jpg — CCR9 expression is highly specific for CD4⁺ and CD8⁺ lymphocytes infiltrating the small intestine. FACS^® analysis reveals that CCR9 is expressed on virtually all CD4⁺ and CD8⁺ lymphocytes in the (A) IEL and (B) LPL compartments of the jejunum. Interestingly, CCR9 was not detected on lymphocytes isolated from (C) normal lung, (D) hepatitis C virus (HCV)-infected liver, (E) breast milk, (F) normal skin, (G) inflamed skin (delayed-type hypersensitivity [DTH]), (H) rheumatoid arthritis (RA) synovium, and (I) rheumatoid arthritis synovial fluid, and (J) a smaller percentage of colon lymphocytes expresses CCR9 compared with jejunal lymphocytes (2,000 cells in each panel). (A–I) Isotype-matched control Ab staining shown as dotted lines. Percentage of CCR9⁺ cells based on a marker encompassing 5% of isotype control–stained cells in both histogram and dot blots. Data are representative of three jejunum, four colon, four liver, two breast milk, four normal skin, four inflamed skin, four synovium, and four synovial fluid samples.