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Jaks are not activated by FL stimulation in Baf3/Flt3 cells. Growth factor–starved Baf3/Flt3 cells were stimulated with FL (100 ng/ml) or IL-3 (10 ng/ml) for 5 min. Jak1, Jak2, Jak3, and Tyk2 were immunoprecipitated from cell lysates and immunoblotted with antiphosphotyrosine antibody (pY). The same membranes were stripped and reblotted with different antibodies as shown in the figure. Results shown are from one representative of three experiments.
