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. 2000 Sep 5;192(5):719–728. doi: 10.1084/jem.192.5.719

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Flt3 receptor expression on the surface of bone marrow cells of Stat5a^+/+, Stat5a^−/−, Stat5b^+/+, and Stat5b^−/− mice as analyzed by flow cytometry. Mouse bone marrow cells were depleted of lineage-positive cells and stained with anti–Flt3–PE and anti–c-kit–FITC antibodies. Large granulocytes and debris were gated out on the basis of their light scatter pattern. The quadrants were set up based on isotype control antibodies. The numbers shown on the two-dimensional dot plots are percentages of single positive cells (Flt3⁺ or c-kit⁺) and double positive cells (Flt3⁺c-kit⁺).

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