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. 2000 Sep 5;192(5):741–754. doi: 10.1084/jem.192.5.741

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Tetramer-positive cells have the expected phenotypic markers of NK T cells. (A) Phenotypic analysis of tetramer-positive cells in the thymus. Histogram overlays of tetramer-positive TCR-β^int cells (open histogram) and control stainings (shaded histogram) reveal the tetramer-positive population to be CD5⁺CD44^highCD69⁺. Conventional T cells served as the control staining for CD44, and for CD5 and CD69, background staining was used due to the presence of conventional T cells staining positive for these markers. (B) Tetramer-positive T cells show a bias in Vβ gene segment usage as compared with conventional T cells. Conventional T cell controls are the lymphocytes expressing high levels of TCR-β in the thymus (upper left gate in A) and TCR-β^high and NK1.1⁻ T cells in the liver. Each of these figures is derived from two experiments with similar results.