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. 2000 Sep 5;192(5):705–718. doi: 10.1084/jem.192.5.705

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JEM000411.f4a.jpg — MIP-3α is selectively produced by epithelial cells. (A) Nonhematopoietic cells, including endothelial cells (human umbilical vein endothelial cells), keratinocytes, and fibroblasts, were seeded at 1–2 × 10⁴ cells/ml, and after 3–5 d of culture (80% confluence), cells were either activated by IL-1β+TNF-α or left unactivated for 48 h. Monocyte-derived DCs and B lymphocytes were either activated by CD40L-transfected L cells (murine fibroblasts, CD40L L cells) or left unactivated for 24 h. Coculture with untransfected L cells was checked for the absence of MIP-3α production. Peripheral blood monocytes and T lymphocytes were activated for 24 h in the presence of LPS and anti-CD3 plus anti-CD28, respectively. IL-1β+TNF-α did not induce MIP-3α production by monocytes or monocyte-derived DCs (not shown). Supernatants were collected and measured for MIP-3α content using a specific ELISA. (B) Keratinocyte supernatants were collected and measured for MIP-3α, MCP-1, MIP-1α, and RANTES contents using specific ELISAs. Results shown are representative of five independent experiments.

graphic file with name JEM000411.f4b.jpg — MIP-3α is selectively produced by epithelial cells. (A) Nonhematopoietic cells, including endothelial cells (human umbilical vein endothelial cells), keratinocytes, and fibroblasts, were seeded at 1–2 × 10⁴ cells/ml, and after 3–5 d of culture (80% confluence), cells were either activated by IL-1β+TNF-α or left unactivated for 48 h. Monocyte-derived DCs and B lymphocytes were either activated by CD40L-transfected L cells (murine fibroblasts, CD40L L cells) or left unactivated for 24 h. Coculture with untransfected L cells was checked for the absence of MIP-3α production. Peripheral blood monocytes and T lymphocytes were activated for 24 h in the presence of LPS and anti-CD3 plus anti-CD28, respectively. IL-1β+TNF-α did not induce MIP-3α production by monocytes or monocyte-derived DCs (not shown). Supernatants were collected and measured for MIP-3α content using a specific ELISA. (B) Keratinocyte supernatants were collected and measured for MIP-3α, MCP-1, MIP-1α, and RANTES contents using specific ELISAs. Results shown are representative of five independent experiments.