Figure 2.

Pool sequencing of peptides eluted from soluble Qa-1^b refolded with a random nonameric library. Recombinant Qa-1^b heavy chain was refolded in the presence of a random nonameric library and human β2m. Free peptide and β2m were removed by gel filtration, and bound peptide was eluted and analyzed by Edman degradation. The yield of each amino acid in pmol (indicated by single letter code) is shown. Positions where the yield of amino acid is at least 150% of the previous cycle are underlined.