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. 2000 Sep 5;192(5):659–670. doi: 10.1084/jem.192.5.659

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JEM992102.f8a.jpg — Analysis of TSLP activity in Whitlock-Witte cultures derived from wild-type or IL-7Rα^−/− mice. (A) Livers from IL-7Rα^−/− or wild-type newborn mice were grown under Whitlock-Witte culture conditions for 9 d in the presence of either IL-7 (10 ng/ml), SCF (1 μg/ml), TSLP (100 ng/ml), or SCF plus TSLP. Enumeration of nonadherent cells was determined by trypan blue exclusion. (B) Immunofluorescent profiles of nonadherent cells isolated from IL-7Rα^−/− and wild-type mice. Lymphoid and myeloid gates were established by the forward versus side light scatter profiles of cells grown in IL-7 alone or SCF alone, respectively. Myeloid gated cells were analyzed for Mac-1 and Gr-1 expression after growth in SCF (gray histogram) or SCF plus TSLP (white histogram). Lymphoid gated cells were analyzed for B220 expression after growth in IL-7 (hatched histogram) or SCF plus TSLP (white histogram). Black histogram represents IgG₁ isotype control staining of cells grown in IL-7.

graphic file with name JEM992102.f8b.jpg — Analysis of TSLP activity in Whitlock-Witte cultures derived from wild-type or IL-7Rα^−/− mice. (A) Livers from IL-7Rα^−/− or wild-type newborn mice were grown under Whitlock-Witte culture conditions for 9 d in the presence of either IL-7 (10 ng/ml), SCF (1 μg/ml), TSLP (100 ng/ml), or SCF plus TSLP. Enumeration of nonadherent cells was determined by trypan blue exclusion. (B) Immunofluorescent profiles of nonadherent cells isolated from IL-7Rα^−/− and wild-type mice. Lymphoid and myeloid gates were established by the forward versus side light scatter profiles of cells grown in IL-7 alone or SCF alone, respectively. Myeloid gated cells were analyzed for Mac-1 and Gr-1 expression after growth in SCF (gray histogram) or SCF plus TSLP (white histogram). Lymphoid gated cells were analyzed for B220 expression after growth in IL-7 (hatched histogram) or SCF plus TSLP (white histogram). Black histogram represents IgG₁ isotype control staining of cells grown in IL-7.