Table 2.
Proliferative Response of Thymocytes and B220+ Cells from γc−/− Mice to TSLP
| γc−/− | γc+/+ | |
|---|---|---|
| cpm × 10−3 | ||
| Thymocytes | ||
| ConA | 4.7 | 7.2 |
| ConA + IL-7 | 4.6 | 25.6 |
| ConA + TSLP 10 ng/ml | 11.5 | 15.3 |
| ConA + TSLP 0.4 ng/ml | 11.2 | 10.9 |
| PMA/ionomycin | 49.5 | 52.4 |
| PMA/IL-4 | 0.2 | 44.1 |
| B220+ cells (RAG-2−/−) | ||
| SCF | 0.2 | 0.5 |
| IL-7 | 0.4 | 11.2 |
| IL-7/SCF | 0.5 | 20.3 |
| TSLP | 1.7 | 1.6 |
| TSLP/SCF | 11.6 | 5.6 |
Enriched B220+ cells are from γc−/−RAG-2−/− and γc+/+RAG-2−/− mice (see Materials and Methods). The values are the arithmetic mean of triplicate wells and are from a single representative experiment.