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IL-4 enhances IL-12 p70 production by mouse splenic DCs. Mouse splenic DCs were cultured with an IL-12 stimulus (LPS, anti-CD40 mAb, poly I:C, CpG, or SAC [10 μg/ml], or a mix of all stimuli together) in the presence of GM-CSF and IFN-γ with or without IL-4, for 18 h. The supernatants were assayed for IL-12 p70 content by ELISA. The result is typical of more than five experiments of this type.
