Table 1.
Lymphoid Subsets in Vav1−/− Mice
| n | wt | Vav1 −/− | |
|---|---|---|---|
| Spleen | |||
| Total lymphocytes | 11 | 653 ± 140 | 671 ± 110 |
| NK1.1+CD3− NK cells | 11 | 17 ± 5 | 25 ± 7 |
| NK1.1+CD3+ NK-T cells | 8 | 9.4 ± 3 | 5.2 ± 2 |
| TCR-αβ+ T cells | 9 | 174 ± 19 | 81 ± 22 |
| IgM+ B cells | 10 | 415 ± 130 | 533 ± 92 |
| Bone marrow | |||
| Total lymphocytes | 5 | 23.4 ± 5.4 | 25.8 ± 6.3 |
| NK1.1+CD3− NK cells | 5 | 0.19 ± 0.02 | 0.37 ± 0.17 |
| Liver | |||
| Total lymphocytes | 7 | 27.4 ± 9.6 | 10.4 ± 1.7 |
| NK1.1+CD3− NK cells | 7 | 1.75 ± 0.5 | 1.41 ± 0.5 |
| NK1.1+CD3+ NK-T cells | 7 | 6.2 ± 0.6 | 1.1 ± 1 |
| Thymus | |||
| Total lymphocytes | 9 | 587 ± 126 | 100 ± 63 |
| NK1.1+CD3− NK cells | 9 | 0.54 ± 0.4 | 0.8 ± 0.4 |
| NK1.1+CD3+ NK-T cells | 9 | 5.0 ± 0.24 | 0.42 ± 0.14 |
Cell suspensions were enumerated and stained with a mixture of mAbs specific for TCR-αβ, IgM, and NK1.1. Absolute cell numbers were calculated based on the percentages of positive cells out of the total number of lymphoid cells. Statistically significant differences (P < 0.05, Student's t test) are indicated in bold type. Splenocyte numbers are expressed in 106, and for the other organs, cell numbers are expressed in 105.