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. 2001 Jun 18;193(12):1403–1412. doi: 10.1084/jem.193.12.1403

Figure 2.

Figure 2

Figure 2

Figure 2

Transfected D10-PcMEGF parasites express a functional MSP-1 chimera. (A) Western blot analysis of parasite proteins from extracted enriched schizont (Schiz) or merozoite (Mer) preparations of parental D10 and the D10-PcMEGF clones (PcMEGF.1 and PcMEGF.2). Proteins were separated by SDS-PAGE under nonreducing conditions, transferred to PVDF membranes, and probed with either 4H9/19 or αPcM19 antibodies as indicated. The position of molecular weight standards are shown to the left and are in kD. (B) Localization of MSP-1 expressed in the transfected lines by indirect IFA. D10-PfM3′ (PfM3′) and D10-PcMEGF.1 (PcMEGF.1) schizont-stage parasites were incubated with a mixture of 4H9/19 and αPcM19 antibodies. After incubation in the presence of a mixture of FITC-conjugated anti–mouse and rhodamine-conjugated anti–rabbit Igs, parasites were visualized by microscopy. Original magnification: 1,000×. The same fields were photographed under fluorescence conditions to detect the FITC or rhodamine fluorochromes. (C) In vitro inhibition assays of D10, D10-PcM3′, and D10-PcMEGF clones (PcMEGF.1 and PcMEGF.2) with different concentrations of αPcM19 antibodies (IgG). Error bars represent SDs.