Figure 1.

Purification of ɛ-TxIX. (A) Elution profile of C. textile venom extract chromatographed on a Sephadex G 50 column. The major Gla-containing peak, ɛ-TxIX (denoted by a horizontal bar), elutes after one column volume (denoted by an arrow). (B) Isolation of ɛ-TxIX (peak denoted by arrow) by HPLC on a C18 column in 0.1% trifluoroacetic acid and eluted with an acetonitrile gradient. The peak denoted by ∗ contains a minor Gla-containing peptide (not analyzed). The material in the other peaks did not contain significant amounts of Gla.