Figure 5.

Influence of TSAd on IL-2 promoter activity. Jurkat cells were transiently transfected with an IL-2 promoter-luciferase reporter construct (A–C) or with NF-κB or NFAT/AP-1-luciferase reporter constructs (C only), a control renilla-luciferase reporter, and constructs encoding the FLAG epitope alone (Control) or FLAG-tagged TSAd (A–C) or FLAG-TSAd (R120K) (B only). After 24 h, cells were activated or not (Mock) with the indicated stimuli for 12 h before determination of luciferase activity. Throughout, data have been normalized to renilla-luciferase counts obtained from mock-stimulated samples and represent the means plus 1 SE of triplicate determinations. In C, the shown data are from the same experiment. The same data shown in A, B, and C have been obtained in five, three, and four independent repeat experiments, respectively.