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. 2001 Jun 18;193(12):1431–1438. doi: 10.1084/jem.193.12.1431

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Failure of transgenic bcl-2 to release postnatal or adult pre-T cells from their γ_c dependence at the CD44^+/−CD25⁺ stage. Lineage marker (CD3, CD4, CD8, B220, Ter119, Mac 1 Gr-1, DX5) negative thymocytes from postnatal day 5 (A–C) or adult (D–F) γ_c wild-type (A and D), γ_c ⁻ (B and E), or γ_c ⁻bcl⁺ (C and F) mice were analyzed by flow cytometry for expression of CD44 vs. CD25. Thymocytes develop from TN 1 (CD44⁺CD25⁻) via TN 2 (CD44⁺CD25⁺) and TN 3 (CD44⁻CD25⁺) to TN 4 (CD44⁻CD25⁻). In γ_c ⁻ mice, thymocyte development is incompletely blocked at the transition from TN 2 to TN 3 causing a strong reduction in TN 4 cells (14% [postnatal] or 21% [adult] TN 4 in γ_c wild-type vs. 1% [for both postnatal or adult] TN 4 in γ_c ⁻). Enforced expression of Bcl-2 does not release postnatal or adult thymic precursors from the developmental block caused by lack of γ_c (compare B vs. C, and E vs. F).