Figure 3.

Caspase-1 and –2 are rapidly activated by invasive Salmonella. Primary bone marrow–derived macrophages isolated from wt mice were infected with wt Salmonella or with the invasion-defective sipB − strain. After 15 min of infection, cells were lysed and the activation state of the caspases was analyzed by immunoblotting. (A) caspase-1; (B) caspase-2; (C) caspase-3; (D) caspase-6; (E) caspase-8; (F) actin, used as a loading control.