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. 2000 Oct 2;192(7):1075–1080. doi: 10.1084/jem.192.7.1075

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Ag-specific proliferative responses and cytokine production by spleen and LN cells from CCR2^+/+ and CCR2^−/− mice. Cells were isolated on days 8, 11, 20, 23, and 26 (spleen) and day 9 (LN; pooled from three mice per group) after disease induction and stimulated in vitro with MOGp35–55. Proliferative responses are shown as stimulation indices for each group responding to Ag concentration of 100 μg/ml. Cytokine concentration was measured from supernatants collected after 48 h (IFN-γ) or 72 h (IL-6) of in vitro culture with peptide (100 μg/ml).