Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Oct 2;192(7):1001–1014. doi: 10.1084/jem.192.7.1001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Spatial organization and function of mitochondria in isolated adult rat cardiac myocytes. (A) Confocal plane of a myocyte loaded with TMRM (125 nM; bar = 5 μm). (B) z-section through A (1-μm resolution). Arrows in A and B denote mitochondria lacking TMRM sequestration. (C) Spontaneous ROS production at sites of low mitochondrial membrane potential: red, TMRM (125 nM) fluorescence; green, DCF (10 μM) fluorescence. (D) Exposure to antimycin A (25 μM) produces widespread numbers of depolarized mitochondria (loss of red TMRM fluorescence) together with increased ROS production (increased green DCF fluorescence). (E) Pretreatment with the ROS scavenger Trolox (2 mM) prevents the antimycin A–induced mitochondrial depolarization seen in D (cell labeled with TMRM and DCF as in D).

Spatial organization and function of mitochondria in isolated adult rat cardiac myocytes. (A) Confocal plane of a myocyte loaded with TMRM (125 nM; bar = 5 μm). (B) z-section through A (1-μm resolution). Arrows in A and B denote mitochondria lacking TMRM sequestration. (C) Spontaneous ROS production at sites of low mitochondrial membrane potential: red, TMRM (125 nM) fluorescence; green, DCF (10 μM) fluorescence. (D) Exposure to antimycin A (25 μM) produces widespread numbers of depolarized mitochondria (loss of red TMRM fluorescence) together with increased ROS production (increased green DCF fluorescence). (E) Pretreatment with the ROS scavenger Trolox (2 mM) prevents the antimycin A–induced mitochondrial depolarization seen in D (cell labeled with TMRM and DCF as in D).