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. 2000 Oct 2;192(7):1001–1014. doi: 10.1084/jem.192.7.1001

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© 2000 The Rockefeller University Press

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(A) Evidence of cooperativity and reversibility of ΔΨ (TMRM) dissipation during 2 Hz line-scan imaging. (B) The abrupt dissipation phase of TMRM fluorescence during line-scan imaging can be described by first-order kinetics. In this example, the dissipation of ΔΨ in a single mitochondrion occurs in step-wise fashion over several seconds. The inset shows all of the segments with negative slope (joined together; after background subtraction), fit by a single exponential model with τ_1/2 = 0.54 s.

(A) Evidence of cooperativity and reversibility of ΔΨ (TMRM) dissipation during 2 Hz line-scan imaging. (B) The abrupt dissipation phase of TMRM fluorescence during line-scan imaging can be described by first-order kinetics. In this example, the dissipation of ΔΨ in a single mitochondrion occurs in step-wise fashion over several seconds. The inset shows all of the segments with negative slope (joined together; after background subtraction), fit by a single exponential model with τ_1/2 = 0.54 s.