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. 2000 Oct 2;192(7):977–986. doi: 10.1084/jem.192.7.977

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IFN-γ signaling disrupts Th1 differentiation in vitro, but does not affect the proliferation of polarized cells. CD4⁺Mel-14^hi T cells were purified from spleens, stimulated with plate-bound anti-CD3 mAbs, and polarized in vitro for 7 d with IL-4 and anti–IFN-γ mAb (Th2) or anti–IL-4 and either IFN-γ or IL-12 (Th1). (A) IFN-γ and (B) IL-4 levels in culture supernatants were determined by ELISA 48 h after restimulation. (C) Proliferation rates of in vitro–differentiated cells were determined after a 6-h pulse with [³H]thymidine. These experiments were performed three times yielding similar results.