Table 1.
Checkerboard Analysis of LL-37–induced Monocyte Migration
| LL-37 in lower wells (μM) | LL-37 in upper wells (μM) | ||
|---|---|---|---|
| 0 | 1 | 10 | |
| 0 | 40 ± 5 | 45 ± 6 | 44 ± 7 |
| 1 | 135 ± 15‡ | 50 ± 8 | 40 ± 6 |
| 10 | 495 ± 29‡ | 265 ± 5‡ | 95 ± 2* |
Human monocytes were used at 106 per ml. LL-37 peptide at the specified concentrations was added to the lower wells of the chemotaxis chamber, and monocytes in the absence or presence of the specified concentration of LL-37 peptide were added to the upper wells of the chemotaxis chamber. The average (mean ± SD) of migrated monocytes of triplicate wells is shown as no./HPF. *P < 0.05 and ‡ P < 0.01 as compared with spontaneous monocyte migration (in bold type).