Table 1.
Sequences of Primers Used for PCR Amplification of cDNA, Product Sizes, and PCR Cycle Numbers
Gene | Primer | Sequence | Size (bp) | Cycle |
---|---|---|---|---|
β-actin | 5′ | 5′-GTCGGGCGCCCCAGGCACCA | 539 | 28 |
3′ | 5′-CTCCTTAATGTCACGCACGATTTC | |||
TNF-α | 5′ | 5′-AACCACCAAGTGGAGGAGCAGC | 312 | 36 |
3′ | 5′-TGACCTCAGCGCTGAGTTGGTCC | |||
IL-1β | 5′ | 5′-TGAAGGGCTGCTTCCAAACCTTTGACC | 322 | 36 |
3′ | 5′-TGTCCATTGAGGTGGAGAGCTTTCAGC | |||
IL-1ra | 5′ | 5′-CCTGCAAGATGCAAGCCTTCAGG | 353 | 35 |
3′ | 5′-CAGCCTCTAGTGTTGTGCAGAGG | |||
IL-4 | 5′ | 5′-GAATGTACCAGGAGCCATATC | 384 | 38 |
3′ | 5′-CTCAGTACTACGAGTAATCCA | |||
TNFR-II | 5′ | 5′-GTAGGCCTTGAGCAGCAGCACCT | 312 | 35 |
3′ | 5′-GTGTCTCTGTAGTCTCACACGG | |||
IL-10 | 5′ | 5′-TCCTTAATGCAGGACTTTAAGGGTTAC | 246 | 38 |
3′ | 5′-GACACCTTGGTCTTGGAGCTTATTAAA | |||
DP receptor | 5′ | 5′-GAAGTTCGTGCAGTACTGTCCAG | 435 | 35 |
3′ | 5′-TCCACTATGGAAATCACAGACAG |