Figure 1.

Intracellular staining for IL-4 and IFN-γ in NKT cells from adult or cord blood stimulated with anti-CD3 and anti-CD28 mAbs. Vα24⁺Vβ11⁺ NKT cells were purified by cell sorting, expanded with anti-CD3 and anti-CD28 mAbs in the presence of PHA, IL-2, and IL-7 for two rounds (14 d each), and restimulated with immobilized anti-CD3 mAb and soluble anti-CD28 mAb at 10⁶ cells per milliliter ml for 5 h. Brefeldin A was added at 2.5 h for intracellular cytokine staining. Percentages in each quadrant are indicated on the plots. The data shown are from one experiment representative of three experiments.