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. 2001 Jan 15;193(2):233–238. doi: 10.1084/jem.193.2.233

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© 2001 The Rockefeller University Press

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Priming of KLH-specific T cells in vivo. (A) Antigen-dependent proliferation. Pre- and postimmunization PBMCs were thawed together and cultured in the absence or presence of KLH (10 μg/ml). Data shown are KLH-specific proliferation after subtracting [³H]TdR incorporation in control wells. SEM for all measurements is <30%. (B) KLH-specific IFN-γ– and IL-4–producing cells from before and after DC immunization were quantified in freshly isolated uncultured PBMCs using an ELISPOT assay. KLH-specific SFCs were calculated after subtracting data from control wells without antigen.

Priming of KLH-specific T cells in vivo. (A) Antigen-dependent proliferation. Pre- and postimmunization PBMCs were thawed together and cultured in the absence or presence of KLH (10 μg/ml). Data shown are KLH-specific proliferation after subtracting [³H]TdR incorporation in control wells. SEM for all measurements is <30%. (B) KLH-specific IFN-γ– and IL-4–producing cells from before and after DC immunization were quantified in freshly isolated uncultured PBMCs using an ELISPOT assay. KLH-specific SFCs were calculated after subtracting data from control wells without antigen.