Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Jan 15;193(2):219–232. doi: 10.1084/jem.193.2.219

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Clonal TCR-β T cell expansion in IL-15tg mice. (a) Schematic of an example rearranged TCR-β gene that was observed in an IL-15tg mouse that died of fatal lymphocytic leukemia. (b) DNA PCR gel showing a monoclonal Jβ2.7 usage in the expanded lymphocytes from an IL-15tg mouse (tg), compared with polyclonal control (C). (c) DNA PCR gel showing a monoclonal Vβ16-Dβ2-Jβ2.7 usage in the expanded lymphocytes from an IL-15tg mouse. A faint band is visible with the prominently used Vβ4, and is present on most gels examining the FVB/N TCR-β. (d) Example of a clonal TCR Vβ6⁺ T cell population in a different IL-15tg mouse with fatal leukemia by flow cytometry. Blood cells were gated on CD3⁺ lymphocytes and assessed for expression of the Vβ6 TCR.

Clonal TCR-β T cell expansion in IL-15tg mice. (a) Schematic of an example rearranged TCR-β gene that was observed in an IL-15tg mouse that died of fatal lymphocytic leukemia. (b) DNA PCR gel showing a monoclonal Jβ2.7 usage in the expanded lymphocytes from an IL-15tg mouse (tg), compared with polyclonal control (C). (c) DNA PCR gel showing a monoclonal Vβ16-Dβ2-Jβ2.7 usage in the expanded lymphocytes from an IL-15tg mouse. A faint band is visible with the prominently used Vβ4, and is present on most gels examining the FVB/N TCR-β. (d) Example of a clonal TCR Vβ6⁺ T cell population in a different IL-15tg mouse with fatal leukemia by flow cytometry. Blood cells were gated on CD3⁺ lymphocytes and assessed for expression of the Vβ6 TCR.