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. 2001 Jan 15;193(2):195–206. doi: 10.1084/jem.193.2.195

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The human HA-8 mHAg epitope is encoded by the KIAA0020 sequence represented in GenBank/EMBL/DDBJ under accession no. D13645. A eukaryotic expression plasmid containing a 669-bp region of the KIAA0020 cDNA encoding RTLDKVLEV was transiently transfected into SDK (mHAg⁻ donor) BLCL as described in Materials and Methods and incubated with the SKH-13 CTL clone in a standard ⁵¹Cr-release assay. SKH is the BLCL of the mHAg⁺recipient.