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. 2001 Jan 15;193(2):195–206. doi: 10.1084/jem.193.2.195

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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HA-8 mHAg allelic segregation analysis. Segregation of the HA-8^R and HA-8^P alleles and correlation of the HA-8^R allele with cytolysis by SKH-13 CTLs at an E/T ratio of 5:1 is shown using the BLCL of CEPH reference family 1362. Filled circles (females) or squares (males) indicate strong lysis by SKH-13 CTLs (phenotype positive); open symbols indicate minimal lysis (phenotype negative). Numbers below symbols were assigned to each family member by the University of Utah. R/P represents HA-8^R/P heterozygous genotype; P/P represents HA-8^P genotype (the HA-8^P/liter polymorphism cannot be distinguished by these genotyping PCR primers). Genotyping of HA-8 mHAg alleles was determined by PCR-RFLP of genomic DNA as described in Materials and Methods.