Figure 6.

HLA-A*0201 binding affinity of HA-8 mHAg peptide variants and SKH-13 CTL recognition of exogenously pulsed peptide variants. (A) Binding of RTLDKVLEV, PTLDKVLEV, and PTLDKVLEL to HLA-A*0201. HPLC-purified synthetic peptides were assayed for their ability to inhibit the binding of the iodinated peptide FLPSDYFPSV (♦) to affinity-purified HLA-A*0201 molecules in a cell-free peptide binding assay (see Materials and Methods). (B) RTLDKVLEV, PTLDKVLEV, and PTLDKVLEL were tested for their ability to reconstitute the epitope for the SKH-13 CTL clone. Epitope reconstitution assay conditions are described in Materials and Methods. An E/T ratio of 10:1 was used. Background CTL lysis of T2 in the absence of any peptides was 4%; positive control lysis (SKH) was 80%.