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. 2001 Jan 15;193(2):195–206. doi: 10.1084/jem.193.2.195

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Comparative SKH-13 CTL recognition of transiently transfected KIAA0020 constructs. (A) CTL recognition of the donor-derived BLCL SDK is shown after transient transfection of experimental minigene constructs. Constructs tested contain a minigene cassette encoding the KIAA0020 polymorphic peptides either with (closed symbols) or without (open symbols) an E3-19K adenovirus leader peptide sequence, which directs translocation of the expressed peptide to the ER, bypassing normal TAP transport. CTL lysis of donor (SDK) and recipient (SKH) BLCL is also shown. (B) Eukaryotic expression plasmids expressing a truncated KIAA0020 protein initiating from the native start codon, encoding RTLDKVLEV, PTLDKVLEV, or PTLDKVLEL without an E3-19K adenovirus leader peptide sequence, were transiently transfected into SDK BLCL as described in Materials and Methods and incubated with the SKH-13 CTL clone in a standard epitope reconstitution assay. CTL lysis of recipient (SKH) and donor (SDK) BLCL cells is indicated.