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. 2000 Nov 6;192(9):1237–1248. doi: 10.1084/jem.192.9.1237

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Nramp1 association with zymosan–FF6-containing phagosomes. Macrophages from normal mice (129sv, +/+; A and B) and from mutant mice with a null mutation at Nramp1 (Nramp1 ⁻ ^/−, −/−; C and D) were harvested and plated on glass coverslips. After 48 h, the cells were allowed to ingest zymosan–FF6 for 1 h at 37°C. The cells were washed free of uningested particles and fixed before indirect immunofluorescence with an affinity-purified rabbit anti-Nramp1 antibody. Phase–contrast images (A and C) and the corresponding immunofluorescence micrographs (B and D) are shown.