Table 5.
Secondary Transplantation of Defective Repopulating Cells
| Percent chimerism | ||||
|---|---|---|---|---|
| Primary recipient | Total | Myeloid | B lymphoid | T lymphoid |
| No. 1 | 88.8 ± 10.8 (n = 5) | 77.5 ± 18.8 (n = 5) | 94.9 ± 8.4 (n = 5) | 90.0 ± 9.0 (n = 5) |
| No. 2 | 51.0 ± 25.8 (n = 10) | 73.4 ± 34.6 (n = 10) | 4.0 ± 5.5 (n = 10) | 21.0 ± 21.3 (n = 10) |
| 80.7 | 97.4 | 11.9 | 55.8 | |
| 68.0 | 91.9 | 14.3 | 10.9 | |
| 58.3 | 80.0 | 1.6 | 2.0 | |
| 55.9 | 71.6 | 1.4 | 61.3 | |
| 50.7 | 93.7 | 8.7 | 20.6 | |
| 12.3 | 12.2 | 1.2 | 19.6 | |
| 78.8 | 93.0 | 0.4 | 22.3 | |
| 53.3 | 98.2 | 0.4 | 3.4 | |
| 50.5 | 89.0 | 0.1 | 13.4 | |
| 1.5 | 7.2 | 0.0 | 0.2 | |
| No. 3 | 9.2 ± 7.9 (n = 6) | 26.2 ± 15.5 (n = 6) | 0.1 ± 0.1 (n = 6) | 0.3 ± 0.2 (n = 6) |
The primary recipients listed as mice Nos. 1, 2, and 3 in Table served as donors for secondary transplantation. Lethally irradiated mice were transplanted with 2 × 106 bone marrow cells per mouse 16 wk after primary transplantation. Between 12 and 16 wk after secondary transplantation, the recipients were analyzed for percent chimerism of CD34−KSL-derived cells.