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. 2000 Nov 6;192(9):1249–1260. doi: 10.1084/jem.192.9.1249

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JEM001194.f3a.jpg — Comparative levels of infectivity in immune cells caused by CTL⁻P⁺ and CTL⁺P⁻ viral variants. In each experiment, four adult BALB/c ByJ mice were injected intravenously with 2 × 10⁶ PFU of virus, and 3 d after infection a pooled splenocyte suspension was prepared from each mouse (see Materials and Methods). Each cell population was labeled with cell-specific monoclonal antibodies to CD4, CD8, B220, CD11c, DEC-205, and CD11b. Subsequent intracellular staining using specific antibody to LCMV NP (antibody 113) was performed, followed by FACS^®. The graph represents percentages of cells in each subset infected with ARM 53b (A) or Cl 13 (B). Summarized below are the percentages of infected antigen-presenting cells (CD11c, CD11b, B220, and DEC-205⁺ cells) or CD4 and CD8 T cells infected with these viruses. The term “other cells” refers to the sum of percentages for infected CD11b⁺, B220⁺, CD4⁺, and CD8⁺ T cells. (A) Infection of cell populations by CTL⁺P⁻ viruses. (B) Infection of cell populations by CTL⁻P⁺ viruses.

graphic file with name JEM001194.f3b.jpg — Comparative levels of infectivity in immune cells caused by CTL⁻P⁺ and CTL⁺P⁻ viral variants. In each experiment, four adult BALB/c ByJ mice were injected intravenously with 2 × 10⁶ PFU of virus, and 3 d after infection a pooled splenocyte suspension was prepared from each mouse (see Materials and Methods). Each cell population was labeled with cell-specific monoclonal antibodies to CD4, CD8, B220, CD11c, DEC-205, and CD11b. Subsequent intracellular staining using specific antibody to LCMV NP (antibody 113) was performed, followed by FACS^®. The graph represents percentages of cells in each subset infected with ARM 53b (A) or Cl 13 (B). Summarized below are the percentages of infected antigen-presenting cells (CD11c, CD11b, B220, and DEC-205⁺ cells) or CD4 and CD8 T cells infected with these viruses. The term “other cells” refers to the sum of percentages for infected CD11b⁺, B220⁺, CD4⁺, and CD8⁺ T cells. (A) Infection of cell populations by CTL⁺P⁻ viruses. (B) Infection of cell populations by CTL⁻P⁺ viruses.