Figure 1.

Previous infection prevents illness during secondary responses to the attachment protein of RSV. (A) Weight loss was monitored on the day of, and during, the final RSV infection. The mean and standard deviation from five to six mice per group are shown. (B) Illness was monitored by a blinded observer using a standard grading system based on the degree of cachexia, ruffled fur, and mobility. The results from five to six mice per group per day were added (cumulative disease severity score). Mice experiencing secondary responses to the G protein are represented by the black bars; those preinfected with RSV and influenza are represented by the gray and white bars, respectively. (C) Eosinophils were enumerated in hematoxylin and eosin–stained cytocentrifuge preparations of cells recovered by BAL. The results represent individual mice from two independent experiments. The groups are designated as follows: A, HEp-2–G-RSV; B, HEp-2–β-gal–RSV; C, RSV–β-gal–RSV; D, Flu–β-gal–RSV; E, RSV–G-RSV; and F, Flu–G-RSV.