Table 3.
Flow Cytometric Analysis of Splenocytes
| B6 | B6/129.Igh b | Cr2 −/− | C4 −/− | |||
|---|---|---|---|---|---|---|
| Age (mo) | 10 | 2 | 10 | 10 | 2 | 10 |
| Percent TCR-β1 | 23.33 ± 2.30 (ns) | 35.45 ± 0.45 | 26.53 ± 1.53 | 29.23 ± 2.01 (ns) | 37.65 ± 3.18 (ns) | 19.06 ± 1.82 (<0.01) |
| Percent B220+ | 55.03 ± 0.96 (ns) | 47.40 ± 2.83 | 55.15 ± 1.15 | 48.98 ± 4.00 | 46.05 ± 3.18 (ns) | 44.16 ± 6.50 (ns) |
| Percent MAC+Gr-1− | 6.85 ± 0.32 (ns) | 7.70 ± 0.84 | 6.09 ± 0.33 | 6.57 ± 0.58 (ns) | 7.07 ± 0.70 (ns) | 11.79 ± 1.12 (<0.005) |
| Percent MAC+Gr-1+ | 2.47 ± 0.23 (<0.05) | 1.86 ± 0.14 | 1.83 ± 0.21 | 2.05 ± 0.22 (ns) | 2.03 ± 0.41 (ns) | 6.57 ± 2.09 (<0.05) |
| Percent TCR-β1CD44high | 13.20 ± 0.87 (ns) | 5.88 ± 1.42 | 12.30 ± 0.24 | 9.65 ± 0.19 (<0.01) | 5.20 ± 1.46 (ns) | 16.58 ± 1.34 (<0.05) |
| Percent TCR-β1GL-7+ | 0.52 ± 0.13 (ns) | 0.26 ± 0.06 | 0.30 ± 0.01 | 0.24 ± 0.03 (ns) | 0.24 ± 0.10 (ns) | 1.56 ± 0.28 (<0.005) |
| Percent TCR-β1Fas+ | 2.04 ± 0.19 (ns) | 0.51 ± 0.09 | 1.62 ± 0.22 | 1.64 ± 0.19 (ns) | 0.56 ± 0.14 (ns) | 7.67 ± 0.93 (<0.0005) |
| Percent B220+CD44high | 1.59 ± 0.18 (ns) | 1.52 ± 0.23 | 1.33 ± 0.09 | 0.91 ± 0.09 (<0.01) | 0.96 ± 0.11 (ns) | 2.34 ± 0.41 (<0.05) |
| Percent B220+GL-7+ | 1.27 ± 0.34 (ns) | 0.45 ± 0.11 | 0.67 ± 0.07 | 0.46 ± 0.10 (ns) | 0.42 ± 0.10 (ns) | 2.63 ± 0.84 (<0.05) |
| Percent B220+Fas+ | 0.31 ± 0.05 (ns) | 0.12 ± 0.01 | 0.23 ± 0.03 | 0.26 ± 0.03 (ns) | 0.13 ± 0.02 (ns) | 0.84 ± 0.14 (<0.005) |
Percentage of TCR-β1, B220+, Mac-1+Gr-1+, or Mac-1+Gr-1− cells are enumerated on a gate of live (7-AAD−) cells. All of the other calculations are on a live lymphocyte gate. P values (Student's t test, compared to age-matched B6/129.Ighb mice) are shown in parentheses; ns, statistically not significant (P > 0.05) in comparison to age-matched B6/129.Ighb mice. Data represent the mean ± SEM from four to five mice at the age of 10 mo and two mice of 2 mo in each group, randomly chosen from two to three independent litters. All mice are female.