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. 2000 Nov 6;192(9):1261–1272. doi: 10.1084/jem.192.9.1261

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The accessibility of L. pneumophila vacuoles to endocytic probes. Macrophages incubated with either formaldehyde-fixed E. coli for 1 h or L. pneumophila (Lp) for the period indicated were incubated with TR-OV for 15 min, then in fresh medium for an additional 5, 30, or 120 min. After fixation and DAPI staining, blinded samples were scored for the percentage of vacuoles that had accumulated detectable TR-OV. Each bar represents the cumulative results from three separate experiments in which 50 vacuoles per condition were scored.