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. 2000 Nov 6;192(9):1261–1272. doi: 10.1084/jem.192.9.1261

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JEM000821.f6a.jpg — The inhibition of L. pneumophila vacuole maturation by BFA. Macrophage lysosomes were labeled either with antibody specific for LAMP-1 or by endocytosis of TR-OV. 1 h after infection, 25 nM BFA was added to the culture medium for the duration of the experiment (open symbols) or was not (filled symbols). (A) In the presence of BFA, L. pneumophila vacuoles failed to merge with the endocytic network (black arrowheads), and each typically contained <15 bacteria. The percentage of 50 vacuoles that colocalized with either (B) LAMP-1 or (C) TR-OV was scored by fluorescence microscopy over the course of a primary infection in three separate experiments, each represented by a different symbol (circles, squares, or diamonds).

graphic file with name JEM000821.f6b.jpg — The inhibition of L. pneumophila vacuole maturation by BFA. Macrophage lysosomes were labeled either with antibody specific for LAMP-1 or by endocytosis of TR-OV. 1 h after infection, 25 nM BFA was added to the culture medium for the duration of the experiment (open symbols) or was not (filled symbols). (A) In the presence of BFA, L. pneumophila vacuoles failed to merge with the endocytic network (black arrowheads), and each typically contained <15 bacteria. The percentage of 50 vacuoles that colocalized with either (B) LAMP-1 or (C) TR-OV was scored by fluorescence microscopy over the course of a primary infection in three separate experiments, each represented by a different symbol (circles, squares, or diamonds).