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. 2000 Nov 6;192(9):1261–1272. doi: 10.1084/jem.192.9.1261

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Acid tolerance of L. pneumophila isolated from macrophages. Macrophages infected with L. pneumophila for 3, 12, or 18 h were lysed, and the bacteria were transferred to broth of pH 5.0 or 6.9. In parallel, E or PE L. pneumophila from pH 6.9 broth were diluted into broth of pH 5.0 or 6.9. After 4 h, samples were plated on CYET to quantify CFU. Percentage survival was calculated as (CFU/ml of AYET, pH 5.0)/(CFU/ml of AYET, pH 6.9) × 100. Means and SE were calculated from four separate experiments.