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. 2000 Nov 6;192(9):1223–1236. doi: 10.1084/jem.192.9.1223

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© 2000 The Rockefeller University Press

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graphic file with name JEM001086.f1ab.jpg — Binding of recombinant wild-type human and mouse ADA to rabbit CD26. (A and B) FPLC assay. Lysates of E. coli SØ3834 cells containing equal units of wild-type human ADA (A) or wild-type mouse ADA (B) were incubated in the absence (open circles) or presence (filled circles) of rabbit CD26, then fractionated on Superose 12. Circles, ADA activity; triangles, DPPIV activity. (C) Nondenaturing PAGE assay. ³⁵S-labeled ADA in vitro translation products were incubated in the presence (+) or absence (−) of rabbit CD26 and then subjected to nondenaturing PAGE, followed by fluorography. Lane 1, wild-type human ADA; lane 2, wild-type human ADA plus rabbit CD26; lane 3, wild-type mouse ADA; lane 4, wild-type mouse ADA plus rabbit CD26.

graphic file with name JEM001086.f1c.jpg — Binding of recombinant wild-type human and mouse ADA to rabbit CD26. (A and B) FPLC assay. Lysates of E. coli SØ3834 cells containing equal units of wild-type human ADA (A) or wild-type mouse ADA (B) were incubated in the absence (open circles) or presence (filled circles) of rabbit CD26, then fractionated on Superose 12. Circles, ADA activity; triangles, DPPIV activity. (C) Nondenaturing PAGE assay. ³⁵S-labeled ADA in vitro translation products were incubated in the presence (+) or absence (−) of rabbit CD26 and then subjected to nondenaturing PAGE, followed by fluorography. Lane 1, wild-type human ADA; lane 2, wild-type human ADA plus rabbit CD26; lane 3, wild-type mouse ADA; lane 4, wild-type mouse ADA plus rabbit CD26.