Table 2.

PCR Primers Used in Constructing Human–Mouse ADA Hybrids

	Primer sequence	Location in ADA cDNA
1	(+)CGCGCGAATTCGGGCACCATGGCCCAGACACCCGCATTC	M1–21 (EcoRI/NcoI)
2	(−)CATCTCTACAAACTCATAGGCGATCCTCTTGATGGCCTCTCTGCA	Reverse of primer 3
3	(+)TGCAGAGAGGCCATCAAGAGGATCGCCTATGAGTTTGTAGAGATG	M223–243/H244–267
4	(−)GCGCAAGCTTCGGGCCATGGTCTTCAGAGGTTCTGCCCTGCAG	H1073–1092 (HindIII/NcoI)
5	(+)CGCGCGAATTCGGGCACCATGGCCCAGACGCCCGCCTTCGAC	H1–24 (EcoRI/NcoI)
6	(−)GTCTCGCTCCCCCTCCTGCAGGCCCTGGCCCACTAGGGCTACCACCT	Reverse of primer 7
	CGTCTGGGGTGACGTCCCCTTCAGTCTGGTTCCAGGG
7	(+)CCCTGGAACCAGACTGAAGGGGACGTCACCCCAGACGAGGTGGTAGC	M346–375/H376–429
	CCTAGTGGGCCAGGGCCTGCAGGAGGGGGAGCGAGAC
8	(−)TGCTTGCTCTCCCTCCTGCAGGCCCTGGTTCACAAGATCCACAACGT	H346–375/M376–429
	CATCAGGGGTGAGGTCCCCTTCAGCCTGGTTCCAGGG
9	(+)CCTGATGACGTTGTGGATCTTGTGAACCAGGGCCTGCAGGAGGGAGA	M376–429/H430–459
	GCAAGCATTCGGGGTCAAGGCTCGGTCCATCCTGTGC
10	(−)GCACAGAATGGACCGGACCTTGATGCCAAAGTCTCGCTCCCCCTCCT	Reverse of primer 11
	GCAGGCCCTGGCCCACTAGGGCTACCACCTCGTCTGG
11	(+)CCAGACGAGGTGGTAGCCCTAGTGGGCCAGGGCCTGCAGGAGGGGGA	H376–429/M430–459
	GCGAGACTTTGGCATCAAGGTCCGGTCCATTCTGTGC
12	(−)TTCTTTCAGTAGTCTGTTGTAGAGGGCCTGGTCTTCCAGGGTGTG	Reverse of primer 13
13	(+)CACACCCTGGAAGACCAGGCCCTCTACAACAGACTACTGAAAGAA	H721–741/M742–765
14	(−)GCGCGATATCCGGGCCATGGTCTCTATTGGTATTCTCTGTAGAGCC	M1037–1059 (EcoRV/NcoI)
15	(−)CATCTCCACAAACTCGTAGGCGATCCTTTTGATAGCCTCCCGGCA	Reverse of primer 16
16	(+)TGCCGGGAGGCTATCAAAAGGATCGCCTACGAGTTTGTGGAGATG	H223–243/M 244–267
17	(−)TTCCTGCCGCAGCCTGTTATAAAGAGCTTCATCCTCGATGGTGTG	Reverse of primer 18
18	(+)CACACCATCGAGGATGAAGCTCTTTATAACAGGCTGCGGCAGGAA	M721–741/H742–765