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. 2000 Nov 6;192(9):1289–1300. doi: 10.1084/jem.192.9.1289

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Trypomastigotes induce [Ca²⁺]_i transients in CHO cells through the B₂R. The changes of [Ca²⁺]_i were determined on CHO-mock or CHO-B₂R preloaded with Fura 2-AM in Ham's F12 medium supplemented with 1 mg/ml of BSA, 12.5 mM Hepes, and 25 μM captopril. The tracings represent cytosolic [Ca²⁺]_i transients that addition of TCTs (parasite/host cell ratio of 10:1) induces in either a single cell or average responses of 20–30 cells. (A) TCTs (arrow) added to CHO-B₂R, average responses. (B) TCTs added to CHO-B₂R that were pretreated for 200 s with 100 nM HOE 140 (left arrow), average responses. (C and E) TCTs added to CHO-B₂R, individual cell response. (D and F) TCTs added to CHO-B₂R pretreated with HOE 140, individual cell responses. (G) TCTs added to CHO-mock, individual cell responses. (H) TCTs added to CHO-mock, average responses. CHO-mock failed to induce significant [Ca²⁺]_i elevations upon BK application (up to 50 nM).