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. 2000 Nov 6;192(9):1373–1380. doi: 10.1084/jem.192.9.1373

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Characterization of anti-hTWEAK mAbs. (A) Inhibition of TWEAK cytotoxicity. Cytotoxic activity of CD8–TWEAK (100 ng/ml) was tested against HSC3 cells in the presence of serially diluted anti-hTWEAK mAbs, CARL-1 (•) or CARL-2 (○), by the WST assay. Data represent mean ± SD of triplicate samples. Similar results were obtained in three independent experiments. (B) Cell surface staining of hTWEAK transfectant. 2PK-3 and hTWEAK/2PK-3 cells were stained with biotinylated CARL-1 or CARL-2, followed by PE-labeled avidin (white histograms). Dark histograms indicate background staining with biotinylated control mAb plus PE-labeled avidin.

Characterization of anti-hTWEAK mAbs. (A) Inhibition of TWEAK cytotoxicity. Cytotoxic activity of CD8–TWEAK (100 ng/ml) was tested against HSC3 cells in the presence of serially diluted anti-hTWEAK mAbs, CARL-1 (•) or CARL-2 (○), by the WST assay. Data represent mean ± SD of triplicate samples. Similar results were obtained in three independent experiments. (B) Cell surface staining of hTWEAK transfectant. 2PK-3 and hTWEAK/2PK-3 cells were stained with biotinylated CARL-1 or CARL-2, followed by PE-labeled avidin (white histograms). Dark histograms indicate background staining with biotinylated control mAb plus PE-labeled avidin.