Figure 7.
Regulatory function of shedding on L-selectin–mediated leukocyte activation. Mac-1 expression on neutrophils, obtained from wt and L−/− mice. (i)Without treatment; (ii) L-selectin cross-linked with MEL-14 F(ab′)2 fragments plus secondary GAR F(ab′)2 fragments; and (iii) L-selectin cross-linked with MEL-14 F(ab′)2 fragments plus secondary GAR F(ab′)2 fragments and blockade of L-selectin shedding. Dotted line, unstained PMNs. Data shown is representative of three independent experiments.