Figure 3.

CFA protection of C57BL/6J mice from pMOG_35–55-induced EAE requires NOS2 expression. (A) C57BL/6J mice (n = 15) were immunized i.p. with 100 μl of CFA containing 50 μg of M. tuberculosis H37Ra. On day 28, these mice, along with a group of nonimmunized C57BL/6J mice (n = 15), were immunized s.c. with 300 μg of pMOG_35–55 to induce EAE. Mice were scored daily for signs of clinical disease. Day numbering in the figure corresponds to days after MOG/CFA immunization. (B) C57BL/6NOS2^−/− mice (n = 15) were pretreated with CFA as above. On day 28, these mice, along with a group of nonimmunized C57BL/6NOS2^−/− mice (n = 15), were immunized s.c. with 300 μg of pMOG_35–55 to induce EAE. Mice were scored daily for signs of clinical disease.