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. 2001 Mar 5;193(5):585–594. doi: 10.1084/jem.193.5.585

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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GRα/GRβ heterodimers are present in neutrophils. The GRα protein in neutrophils and PBMCs were immunoprecipitated with a GRα-specific antibody, then Western blotted with an anti-GRβ–specific antibody. PBMCs showed weak bands indicating low levels of heterodimers (lanes 1–4). In contrast, neutrophils demonstrated strong GRβ staining, indicating a high level of GRα/GRβ heterodimers (lanes 5–8).