Table 1.

Analysis of Lymphocyte Subsets in Cockroach Allergen-challenged Lungs of Control and CCR8^−/− Mice

Group	Total cells (107)	CD4+ (106)	CD8+ (106)	CD19+ (106)
CCR8+/+	2.2 ± 0.16	1.2 ± 0.8	0.50 ± 0.2	0.8 ± 0.2
CCR8−/−	1.6 ± 0.18	1.8 ± 0.14	0.58 ± 0.3	1.3 ± 0.14
P	0.015	>0.05	>0.05	>0.05

Cockroach antigen sensitization and challenge was performed as described in Materials and Methods. Lungs were collected and dispersed 24 h after the final intratracheal challenge, and then subjected to flow cytometric analysis. Values are means ± SD derived from four individual mice. The reduction in total cell numbers in CCR8^−/− was due to decreased eosinophil recruitment (CCR8^+/+, 25.5 ± 5% versus CCR8^−/−, 14 ± 4%). Control and knockout mice were on inbred 129 background.