Figure 1.

Residual αβ T cell subsets in MHC-versus MHC/CD1d–deficient mice. (A) NK1.1⁺ and NK1.1⁻ subsets. FACS^® dot plots represent the TCR-β¹ gated splenocytes from MHC class II– and MHC class I–deficient mice stained with antibodies against NK1.1, CD4, and/or CD8. In the bar graphs, bars represent the average ratio of the numbers of each cell type in CD1d^−/− mice versus CD1d^+/− littermates (5–14 littermate pairs per data point). MHC class I^−/− mice were either K^bD^b−/− or TAP^−/−; results with K^bD^b−/− mice (shown in the FACS^® dot plots) and TAP^−/− mice (summarized in the bar graphs) were similar. Error bars are not shown for graphic clarity. ND, not detected (frequency <0.05%). *Statistically significant (P < 0.05). (B) CD1d/αGalCer⁺ and CD1d/αGalCer⁻ subsets. The FACS^® dot plots show the CD4 and CD1d/αGalCer tetramer profiles of TCR-β¹ gated cells from MHC class II^−/−CD1d^+/− and MHC class II^−/−CD1d^−/− littermates, as indicated. The bar graphs represent the average ratio of the numbers of CD1d/αGalCer⁻ cells in CD1d^−/− mice versus CD1d^+/− littermates (four to eight littermate pairs per data point).